uropathogenic e coli cft073  (ATCC)

Journal: Scientific Reports

Article Title: Biophysical and transcriptomic characterization of LL-37-derived antimicrobial peptide targeting multidrug-resistant Escherichia coli and ESKAPE pathogens

doi: 10.1038/s41598-025-22890-7

Figure Lengend Snippet: Antimicrobial efficacy of the peptide against various Escherichia coli strains. (A) Minimum inhibitory concentrations (MICs) determined for laboratory E. coli K-12, uropathogenic E. coli (UPEC), and multidrug-resistant (MDR) strain, following CLSI guidelines. Data are shown as mean ± SD from three independent biological replicates, with error bars indicating SD. (B) Time-kill assays showing bactericidal kinetics at four times the MIC concentration, with 90% bacterial reduction achieved within 2 h for all strains. Values represent mean CFU reduction rate ± SD from three independent replicates; error bars indicate SD. (C) Assessment of resistance development after 10 serial passages of each strain in sub-MIC peptide concentrations, indicating no significant increase in MIC and low potential for resistance emergence. MIC values are normalized to passage 1 baseline and presented as mean ± SD from three independent replicates, with error bars indicating SD.

Article Snippet: Bacterial strains used in this study included E. coli K-12 (ATCC® 10798TM, ATCC), uropathogenic E. coli CFT073 (ATCC® 700928TM, ATCC), multidrug-resistant (MDR) E. coli NCTC 13353TM (NCTC), Pseudomonas aeruginosa (ATCC® 27853TM, ATCC), Acinetobacter baumannii (ATCC® 19606TM, ATCC), Klebsiella quasipneumoniae (ATCC® 700603TM, ATCC), Staphylococcus aureus (ATCC® 25923TM, ATCC), and Enterococcus faecium (ATCC® 19434TM, ATCC).

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: Biophysical and transcriptomic characterization of LL-37-derived antimicrobial peptide targeting multidrug-resistant Escherichia coli and ESKAPE pathogens

doi: 10.1038/s41598-025-22890-7

Figure Lengend Snippet: Mechanism of membrane disruption and transcriptomic response to peptide treatment in E. coli strains. (A) Calcein dye leakage assay demonstrating selective membrane permeabilization of bacterial-mimetic POPE/POPG vesicles by the peptide, with minimal leakage observed in mammalian-mimetic POPC/cholesterol vesicles. Values expressed as % dye leakage relative to 0.1% Triton X-100 (100% release) and shown as mean ± SD from three independent experiments, with error bars indicating SD. (B) RNA sequencing analysis showing upregulation of stress response and efflux pump genes and downregulation of growth-related genes in E. coli K-12, UPEC, and MDR strains after sub-MIC peptide exposure. Data presented as log₂ fold change relative to untreated control; mean ± SE of three biological replicates, with error bars indicating SE. (C) KEGG pathway enrichment analysis highlighting activation of stress response and protein folding pathways alongside repression of DNA replication and cell division pathways across the three E. coli strains. Data represent mean enrichment scores ± SD from three replicates, with error bars indicating SD; enrichment significance adjusted by Benjamini–Hochberg correction (FDR < 0.05).

Article Snippet: Bacterial strains used in this study included E. coli K-12 (ATCC® 10798TM, ATCC), uropathogenic E. coli CFT073 (ATCC® 700928TM, ATCC), multidrug-resistant (MDR) E. coli NCTC 13353TM (NCTC), Pseudomonas aeruginosa (ATCC® 27853TM, ATCC), Acinetobacter baumannii (ATCC® 19606TM, ATCC), Klebsiella quasipneumoniae (ATCC® 700603TM, ATCC), Staphylococcus aureus (ATCC® 25923TM, ATCC), and Enterococcus faecium (ATCC® 19434TM, ATCC).

Techniques: Membrane, Disruption, RNA Sequencing, Control, Activation Assay

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: Analysis of the prevalence of PAIs (A) and the number of PAIs (B) among commensal Escherichia coli from adults and young children. *Statistically significant.

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: Analysis of the relative expression level of the siderophore receptor genes fyuA (A) , iutA (B) , iroN (C) , ireA (D) among Escherichia coli isolates from adults and young children. E. coli 85—an isolate of commensal E. coli from our collection, showing expression levels for the remaining genes ( fyuA , iutA , and iroN ) similar to the control UPEC strains—was used as a control for normalization.

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques: Expressing, Control

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: Distribution of the STs among commensal Escherichia coli isolates from adults (A) and young children (B) .

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: Associations between ST and relative expression of siderophore receptor genes fyuA , iutA , iroN , and ireA among Escherichia coli isolates from adults.

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques: Expressing

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: A maximum-likelihood tree depicting the phylogenetic relationships based on the nucleotide sequences of seven housekeeping genes: adk , fumC , gyrB , icd , mdh , purA , and recA among the Escherichia coli isolates from adults (A) and young children (B) . The tree was reconstructed with RAxML, using a general time-reversible nucleotide substitution model (GTRGAMMA) and 1,000 bootstrap replicates. The isolates’ names are indicated at the tip of the tree. Next, the phylogroups with the color displayed in the legend, STs, and clonal complexes are presented. A parts heat map is given on the right side of the figure. The first part, designated as genotype, encompasses PAIs and the VGs clustered according to their function and marked with different colors displayed in the legend. The second part, designated as phenotype, presents phenotypic traits of virulence, marked with colors corresponding to the genotype in the legend. The degree of biofilm formation was classified into three categories: S, strong; M, moderate; and W, weak.

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Antifungal, anti-biofilm, and anti-hyphal properties of N -substituted phthalimide derivatives against Candida species

doi: 10.3389/fcimb.2024.1414618

Figure Lengend Snippet: Impact of N -butylphthalimide (NBP) on the biofilm formation and planktonic cell growth of Candida species. By cultivating for 24 h at 37°C under static conditions in 96-well polystyrene plates, the planktonic cell growth of Candida albicans DAY185 (A) and the anti-biofilm activities of NBP against C. albicans 10231 (B) , Candida parapsilosis (C) , Staphylococcus epidermidis (D) , Vibrio parahaemolyticus (E) , uropathogenic Escherichia coli (F) , Staphylococcus aureus ATCC 6538 (G) , and polymicrobial biofilms (H) were investigated. *p<0.05 vs untreated controls (None).

Article Snippet: The uropathogenic E. coli (UPEC) O6:H1 strain CFT073 (ATCC 700928) was streaked on Luria–Bertani (LB) agar and incubated in nutrient broth at 37°C for 12 h for overnight culture.

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